|IHPE UMR 5244
University of Perpignan via Domitia
52 Avenue Paul Alduy
F-66860 Perpignan Cedex – FRANCE
email : email@example.com
POSITION : Ph.D student
THESIS DIRECTORS : Christoph GRUNAU & David DUVAL
TEAM : ECOEVI
THESIS SUBJECT :
Genome-wide comparison of chromatin structure changes in infective forms of parasitic flatworms
KEY WORDS :
epigenetics, Stem cells, developpement, Schistosoma mansoni, Ecchinococcus multilocularis, Chip-seq, bioinformatics
THESIS PROJECT :
My thesis project consist to study the chromatin structure in stem cells of two parasitic flatworms: Schistosoma mansoni and Echinococcus multilocularis. These two parasites are in re-emergence situation in Europe. They have a complex life cycle (see Figure 1) comprising many stages during their cycles and two different strategies emerge. Indeed, in the first, the miracidia and cercariae larvae search actively their host. For the second parasite, the larvae are waiting passively their hosts, which accidentally ingest them and the parasite continues the cycle. Moreover the stage of metacestode Ecchinococcus is filled stem cells. These stems cells were also identified at all stages of their cycles. For study their chromatin structures, we will use Chip-seq and RNA-seq. This work will be realised with a collaboration of Klaus Brehm, University of Wurzburg (Germany).
Fig. Schistosoma mansoni and Ecchinococcus multilocularis life cycle.
PUBLICATIONS BEFORE THIS LAB
– Boutte J., Aliaga B., Lima O., Ferreira de Carvalho J., Ainouche A., Macas J., Rousseau-Gueutin M., Coriton O., Ainouche M., Salmon A. 2016. Haplotype detection from next generation sequencing in high ploidy-level species: 45S rDNA gene copies in the hexaploid Spartina maritima. G3 Genes Genomes Genetics, 6: 29-40
- Aliaga B. Genome-wide comparison of chromatin structure changes in infective forms of parasitic flatworms . 6th Day of the Network IMH « Interaction Micro-organism-Hosts », November 2014, Montpellier France
– J. Boutte, B. Aliaga, J. Ferreira de Carvalho, O. Lima, S. Coudouel, D. Naquin, P. Wincker, J. Poulain, C. Da Silva, M. Ainouche and A. Salmon. Automatized detection of duplicated copies from NGS data: application to 45S rDNA and coding genes in hexaploid Spartina species (Poaceae). International Conference on Polyploidy, Hybridization, and Biodiversity (ICPHB), May 2012, Průhonice, Czech Republic.