IHPE – BMC english

TEAM BMC
Molecular Basis of Co-evolution
Head : Christoph GRUNAU

Our group is particularly interested in host / parasite and host / symbiont interactions. We work on two models: (i) Schistosoma mansoni (the agent of intestinal bilharziosis) and its intermediate host, different species of Biomphalaria snails, and (ii) the tropical coral Pocillopora damicornis and it’s symbionte, symbiodinium. Our aim is to identify the molecular mechanisms that are the bases for parasite/symbiont infection success and host defense. Our approaches are based on the idea that colonization of the host (“host compatibility”) is possible for “matching phenotypes”.
Matching phenotypes have molecular bases that can be studied on two levels: first the functional level on which an interaction occurs between the defense systems of the host and the molecules of the pathogene/symbiont that are produced to counteract these. A special emphasis of our work lies therefore on acquired and innate immunity of invertebrates. A second study level concerns the heritable information that is used to code for compatible and incompatible molecular phenotypes. Heritable phenotypic variants that are the basis for host-parasite/symbiont co-evolution can be caused by changes in the genetic and/or epigenetic information and a major goal of our research is to characterize the relative importance of these both systems in rapidly evolving biological systems.
The ultimate goal of our project is to elucidate the relation between environment, epigenetic and genetic diversity/variability and fitness (infection success, resistance to bleaching) in the field and to develop new approaches to control and monitor host-parasite/symbiont interactions.

- Postdoctoral Fellow Schistosoma epigenetics. Deadline end of april (Informations)
- Workshop CEMEB « Epigenetics in Ecology and Evolution » (Montpellier, France) 04/04/2016
- Thematical School CNRS  « BioPerspectives » (Cargèse, Corse) 03/29 to 04/02/2016
- Workshop « mollusk epigenetic » (Montpellier, France) 02/02/2016 (! organizers !)
- ESEB 2015 (Lausanne, Swiss) 08/10-14/2015
Wellcome trust Grant:
Press release with the  CNRS 06/23/2015 « 5 Millions Euros allowed to an international consortium to fight against parasitic worms »
- SMBE 2015 (Vienna, Austria) 07/12-16/2015
- JOBIM 2015 (Clermont Ferrand, France) 07/6-9/2015
- International Workshop « Perpignan’s days on Applied Mathematics » (Perpignan, France) 06/10-12/2015 pdf of the programm
- Thematical School « Eco-Evo-Epigen » (St.Pierre d’Oléron, France) 05/18-22/2015 (! organizers !)

-Annual Meeting of the Network REID « Ecologie des Interactions Durables » (Lyon, France) 03/23–26/2015

- Invited speaker in « Epigenetics and health » program for teenagers science professors (Montpellier, France) 02/2015
- Conference Leibniz Institute for Age Research (Jena, Germany) 04/16/2015

PERMANENT STAFF:
GRUNAU Christoph Professor University of Perpignan (100%)
GOURBAL Benjamin Assistant Professor University of Perpignan (100%)
COSSEAU Céline Assistant Professor University of Perpignan (100%)
DUVAL David Assistant Professor University of Perpignan (100%)
GALINIER Richard Engineer CNRS (100%)
NON PERMANENT STAFF:
Postdoctoral Fellow
TETREAU Guillaume, Postdoctoral Fellow, University of Perpignan
Ph.D students
ALBA Annia, Ph.D student University of Perpignan & University La Havane
- ALIAGA Benoît, Ph.D student University of Perpignan
PINAUD Silvain, Ph.D student University of Perpignan
PORTET Anaïs, Ph.D student University of Perpignan
Guests
COUTINHO CARNEIRO Vitor, PostDoctoral Fellow, Instituto de Bioquimica Medica de l’Universidade do Brasil, BRASIL
FNEICH Sara, PostDoctoral Fellow, INRA, Jouy-en-Josas, FRANCE
BULLA Ingo, researcher, institut für Mathematik und Informatik, Universität Greifswald, GERMANY
- PRATX Loris, PhD, INRA Institut Sophia Agrobiotech, Sophia Antipolis, Nice, FRANCE
GOMEZ-DIAZ Elena, PhD, Donana Biological Station, Sevilla, SPAIN
- SENTIS Arnaud, PostDoctoral Fellow, Laboratory Evolution and Biological Diversity EDB, University of Toulouse III, Toulouse FRANCE
RONDON Rodolfo, Ph.D student, team MIMM, Ifremer
DE CARVALHO AUGUSTO Ronaldo, Ph.D student, Laboratorio de Avaliaçao e Promocao da Saude Ambiental, Rio de Janeiro, BRASIL
FORMER COLLABORATORS…
ROQUIS David, PhD student (2012-2015), Postdoctoral fellow,  laboratory « Population Epigenetics and Epigenomics » , Technischse Universität München, Munich, Germany email
- LIMA Mariana, Ph.D student (march-september 2015), Universidade Federal Rural do Rio de Janeiro, BRASIL email
BOON Nele, Ph.D student (april-august 2015), Katholieke Universiteit Leuven, BELGIUM
- DHEILLY Nolwenn, PostDoctoral Fellow (2011-2013) Assistant Professor, Stony Brook University, NY, USA
FNEICH Sara, Ph.D Student (2011-2014) Postdoctoral Fellow, INRA, UMR 1198 Biologie du Développement et de la Reproduction, Jouy en Josas, FRANCE email
PORTELA Julien, Ph.D student ( 2010-2013) creator of the start-up « GeneLife »
LEPESANT Julie, Ph.D Student (2009-2012) Postdoctoral Fellow, Foundation ARC for Cancer Research, Toulouse, FRANCE email
MONE Yves, Ph.D Student (2007-2010) Postdoctoral Fellow, INRA Montpellier, FRANCE
AFFOURMOU Kouamé, Ph.D Student (2007-2009) Assistant Professor, University of  Cocody, IVORY COAST email
ROGER Emmanuel, Ph.D Student (2004-2008) Assistant Professor, University of Lille, FRANCE email

Protocols in molecular biology and epigenetics © BMC


General Methods

Epigenetics

Bio-Informatics

Cell lines

  • HT1080: human fibrosarcoma culture (male), epithelial-like adherent cells growing as monolayers
  • TOU: lymphoblast cell culture, immortalized by transformation with EBV, contains 21p-

Schistosoma mansoni

NATIONAL COLLABORATIONS :
- P.B. ARIMONDO, Lab Pierre Fabre Centre de Recherche & Développement, Toulouse
– P. ABAD & C. COUSTAU, INRA Sophia-Antipolis, Nice
– R. PIERCE & E. ROGER, U 547 Inserm-Université Lille 2-Institut Pasteur, Lille
– J-M. REICHHART, Institut de Biologie Moléculaire et Cellulaire, CNRS, Strasbourg
– Y. MORET, BioGéoSciences, CNRS, Université de Bourgogne, Dijon
INTERNATIONAL COLLABORATIONS :
– S. MAS-COMA, Dept. Parasitologia, Universidad de Valencia, Spain
– L. DU PASQUIER, Université de  Basel, Switzerland
– T. HUYSE, Royal Museum for Central Africa, Tervuren, Belgium
– N. BOON, Laboratory of Biodiversity and Evolutionary Genomics, Leuven, Belgium
– M. REICHELT, MPI CE, Jena, Germany
– J. BULLA, Dpt. Mathématiques, Université de Bergen, Norway
– M. FREITAG & M. BLOUIN, Oregon State University, Corvallis, USA
– C. ADEMA, Center for Evolutionary and Theoretical Immunology, University of New Mexico, USA
– T. ANDERSON, Texas Biomedical Research Institute, USA
– G. OLIVEIRA, Centro de Pesquisas Rene Rachou, Belo Horizonte, Brazil
– O. NOYA, Instituto de Medicina Tropical, Universidad Central de Venezuela, Caracas, Venezuela

- CUNIPAT (2015-2018) – AgriBio4 / INRA –
Analyse et conception de modes de gestion intégrés (pâturage, production, santé animale) en systèmes cunicoles AB. Analyse de la résistance aux parasites via l’ingestion de tannins chez le lapin.
- TRANSGIGAS (2016-2019) – Chercheur d’Avenir Région LR –
Acquisition de résistances TRANS générationnelles chez l’huître creuse Crassostrea GIGAS : implication de la composante épigénétique
-  FUGI (2015-2020) – Wellcome Trust Strategic Award – link
Functionnal genomics fo flatworms – work-package epigenetic analysis
-  MATER-IMMUNITY (2014-2019) – ANR –
Transfert maternel d’immunité chez les insectes / Caractérisation fonctionnelle et évolution
- INVIMORY (2014-2018) – ANR –
Immunité mémoire chez les invertébrés : spécificité et mécanismes de l’immunité mémoire chez le mollusque Lophotrochozoaire Biomphalaria glabrata
 

PREVIOUS GRANTS

- AURORA (2015)  – Campus France (collaboration Norway)
Statistical modelling in Epigenetics: a pan-species study of distribution of CpG o/e ratios and DNA methylation patterns
- EUPHORBIA (2015) – CNPq Brésil –
Gene expression in the Schistosoma mansoni exposed to Euphorbia milii latex
- EPIGEVOL (2010-2014) – ANR –
Mécanismes épigénétiques et génétiques de l’évolution adaptative: les parasites métazoaires peuvent-ils l’emporter sur la Reine Rouge ?

DISCLAIMER – Our group provides this data in good faith, but makes no warranty, express or implied, or assumes any legal liability or responsibility for any purpose that the data is used for.


MethDB:  the database for DNA methylation and environmental epigenetic effects.


PrimerDB:  the local oligonucleotide database (access restriction applies).


AntibodyDB:  the local antibody database (access restriction applies).


Local Galaxy Instance provides a graphical interface for bioinformatics analysis (access restriction applies)


SchistoGbrowse v2.54 genome browser for assembly version 5.2 (access restriction applies)


SnakeCharmer for selection of restriction enzymes for COmbined Bisulfite Restriiction Assay (COBRA)


MethTools analysis of bisulfite DNA methylation sequencing